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Human Immunoglobulin G3 (IgG3)
Native, IgG subclass 3 whole molecule purified from multiple donor native IgG Polyclonal
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Proteins Synonyms: IgG3 Source: Human Plasma Purity: ≥ 95% by SDS-PAGE Physical State: Frozen in 20 mM phosphate, pH 7.4, with 150 mM NaCl and 0.05% NaN3. Manufacturer: |
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Athens Research & Technology
Produktdetails
Synonyms
IgG3
Description of Human Immunoglobulin G3 (IgG3)
Human Immunoglobulin G3 (IgG3) is a 170 kDa protein. Serum IgG3, constituting ~6% of total IgG, is distinguished by an elongated 62-amino-acid hinge region stabilized by 11 disulfide bonds, conferring unmatched flexibility for antigen engagement and effector function activation. This subclass exhibits potent complement activation via C1q binding and high-affinity interactions with FcγRI/III receptors, surpassing IgG1 in triggering antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis. Its extended hinge enhances spatial tolerance for antigen binding, enabling superior neutralization of enveloped viruses like HIV and CHIKV. IgG3 deficiency (<0.3 g/L) correlates with Wiskott-Aldrich syndrome, systemic lupus erythematosus (SLE), and recurrent bacterial/viral infections due to impaired immune complex clearance. In SLE models, IgG3 deposits drive glomerulonephritis via FcγR-mediated neutrophil activation, while its deficiency attenuates renal damage. Paradoxically, early IgG3 responses in acute infections (e.g., CHIKV) correlate with severe initial symptoms but prevent chronic arthralgia by rapid viral clearance. Clinically, IgG3’s short half-life (7 days vs. IgG1’s 21 days) stems from Arg435-mediated FcRn competition, though the H435 allotype extends half-life for therapeutic use. Anti-HIV IgG3 antibodies peak within 4–5 months post-infection, serving as transient biomarkers for recent exposure6. Hinge-length polymorphisms (3 vs. 4 repeats) influence COVID-19 severity, with shorter hinges impairing viral neutralization and increasing mortality risk. Engineered IgG3 variants with stabilized hinges enhance phagocytosis 3-fold, offering promise for antibody therapeutics targeting intracellular pathogens.
Source
Human plasma non-reactive for HBsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests
Storage
For long term, store Human Immunoglobulin G3 (IgG3) at +2 to 8°C.
Applications
Inflammation, Biotherapeutics, In Vitro Diagnostic, Wiskott-Aldrich syndrome, Autoimmune Diseases, Infection.
Citations/Publications
Devaux, J. J., et al., (2016), 'Neurofascin-155 IgG4 in chronic inflammatory demyelinating polyneuropathy', Neurology, 86(9): pp 800-7. Available at: doi: 10.1212/WNL.0000000000002418.
Sun, X., et al., (2018), 'Identification and Characterization of Novel Fc-Binding Heptapeptides from Experiments and Simulations', Polymers 10: pp 778. Available at: doi:10.3390/polym10070778
Kruljec, N., et al., (2018), 'Development and Characterization of Peptide Ligands of Immunoglobulin G Fc Region', Bioconjugate Chem. 29: pp 2763−2775. Available at: DOI: 10.1021/acs.bioconjchem.8b00395
Bozovičar, K., et al., (2021), 'Focused peptide library screening as a route to a superior affinity ligand for antibody purification', Scientific Reports 11: pp 11650. Available at: https://doi.org/10.1038/s41598-021-91208-0
Shah, B., et al., (2022), 'Observation of Heavy-Chain C-Terminal Amidation in Human Endogenous IgG', Journal of Pharmaceutical Sciences 111: pp 2445−2450. Available at: https://doi.org/10.1016/j.xphs.2022.06.012
Shipped with ice packs
Gel Scan of Human Immunoglobulin G3 (IgG3)
| Fig. 1: SDS-PAGE Gel |
Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.
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Payment Methods
Paying by bank transfer
