Human MIF recombinant Protein

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Phone: +49 (0) 40 . 43 20 84 48 0

E-Mail: info@active-bioscience.de

 

Product Overview, Sizes and Prices

 

Protein

Synonyms: Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, MMIF, MIF.

Source: E. coli

Purity: ≥ 97 % (SDS-PAGE, RP-HPLC)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1701.950.010 10 µg 65 €
1701.950.100 100 µg 0 €
1701.950.199 1 mg 2.390 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, MMIF, MIF.

 

Description of Human MIF

MIF human Recombinant was cloned into an E.coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.Macrophage Inducing Factor Human Recombinant is a single, non-glycosylated, polypeptide chain containing 115 amino acids and having a molecular mass of 12.5 kDa.

 

Biological Activity

Human PBMCs were cultured with 0 to 1000ng/ml Human MIF. Production of IL-8 was measured via ELISA after 24 hours. The ED50 which was found to be 88-132ng/ml.

 

Reconstitution

It is recommended to reconstitute the lyophilized MIF-Protein in sterile 18MO-cm H2O at a concentration between 0.1mg-1mg per 1ml.

 

Stability

Lyophilized MIF-protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MIF-protein should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.

 

Amino Acid Sequence

MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA

 

 

 

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Errors, changes and availability excepted.

 

 

Payment Methods

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Shipping

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Free shipping for orders above 500 € within Germany

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