Human/Murine/Rat BDNF recombinant Protein

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Product Overview, Sizes and Prices
	Protein Synonyms: Brain-Derived Neurotrophic Factor, BDNF, MGC34632. Source: E. coli Purity: ≥ 97 % (SDS-PAGE, RP-HPLC) Physical state: Lyophilized Manufacturer:  Active Bioscience	Article No. Size Price   1185.950.002 2 µg 60 € 1185.950.010 10 µg 159 € 1185.950.050 50 µg 450 € 1185.950.100 100 µg 780 € 1185.950.199 1 mg 3990 € Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

Brain-Derived Neurotrophic Factor, BDNF, MGC34632.

 

Description of Human/Murine/Rat BDNF

Brain-derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids (and an N-terminal Met) and having a total molecular mass of 27kDa. BDNF Human Recombinant is purified by proprietary chromatographic techniques.

 

Biological Activity

The ED₅₀, as determined by the dose-dependent induction of C6 cells proliferation, is 1.3-2µg/ml.

 

Reconstitution

It is recommended to reconstitute the lyophilized BDNF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 

Stability

Lyophilized BDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BDNF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.

 

Amino Acid Sequence

MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR

 

 

 

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