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Human Noggin (glycosylated, InCs) recombinant Protein
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Protein Synonyms: SYM1, SYNS1, NOG. Source: Insect cells Purity: ≥ 95 % (SDS-PAGE, RP-HPLC) Physical state: Lyophilized Manufacturer: |
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Active Bioscience
Product Details
Synonyms
SYM1, SYNS1, NOG.
Description of Human Noggin (glycosylated, InCs)
Noggin produced in Sf9 Baculovirus cells is a glycosylated homodimer containing 205 amino acids and having a molecular mass of 47.9kDa under non-reducing conditions. (Molecular size on SDS-PAGE will appear at approximately 50-80kDa).
Biological Activity
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cellsans was fount to be 0.04-0.2 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4.
Reconstitution
It is recommended to reconstitute the lyophilized Noggin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Noggin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Noggin should be stored at 4°C between 2-7 days and for future use below -18°C. Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC
Shipped at room temperature
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Payment Methods
Paying by bank transfer
