EN
Human Proteinase 3 (PR3)
Native whole molecule
Product Overview, Sizes and Prices | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Proteins Synonyms: PR3, PRTN3, Myeloblastin, Wegener autoantigen, AGP7, P29, C-ANCA antigen, MBN Source: Human Neutrophils Purity: ≥ 95% by SDS-PAGE. Physical State: Frozen in 50 mM MES, pH 4.5 with 700 mM NaCl Manufacturer: |
|
|||||||||||||||||||||
Athens Research & Technology
Produktdetails
Synonyms
PR3, PRTN3, Myeloblastin, Wegener autoantigen, AGP7, P29, C-ANCA antigen, MBN
Description of Human Proteinase 3 (PR3)
Human Proteinase 3 (PR3) is a 29 kDa protein. Proteinase 3 (PR3) is a serine protease predominantly stored in the azurophilic granules of neutrophils and monocytes, where it contributes to the degradation of extracellular matrix proteins, pathogen destruction, and regulation of inflammation. Beyond its enzymatic activity, PR3 is notable as the primary autoantigen in granulomatosis with polyangiitis (GPA, formerly Wegener’s granulomatosis), a severe autoimmune small-vessel vasculitis. In GPA, autoantibodies known as PR3-ANCA (anti-neutrophil cytoplasmic antibodies) target PR3, leading to inappropriate activation of neutrophils, which then release inflammatory mediators and cause vascular injury and granulomatous inflammation in organs such as the respiratory tract and kidneys. PR3 is also expressed on the surface of neutrophils, especially during activation and apoptosis, where it can modulate immune responses and influence microvesicle production, further amplifying inflammation. Clinically, detection of PR3-ANCA is a key diagnostic marker for GPA and helps differentiate it from other vasculitides. Research into PR3’s structure and membrane interactions is ongoing, with the aim of developing targeted therapies for autoimmune vasculitis and understanding PR3’s broader roles in immunity and inflammation.
Source
Neutrophils shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
Storage
For long term, store Human Proteinase 3 (PR3) at ≤ -80°C.
Applications
In Vitro Diagnostics, Vasculitis, Autoimmune Diseases, Antigen Production, Assay Standards, Infection, Inflammation, Cancer.
Citations/Publications
Zheng-Gérard, C., et al., (2022), 'GPR97 triggers inflammatory processes in human neutrophils via a macromolecular complex upstream of PAR2 activation.', Nature Communications. 13: pp 6385. Available at: https://doi.org/10.1038/s41467-022-34083-1
Li, Y., et al., (2018), 'Cystine-knot peptide inhibitors of HTRA1 bind to a cryptic pocket within the active site region', Nature Communications 15: pp 4359. Available at: https://doi.org/10.1038/s41467-024-48655-w
Alatrash, G., et al., (2018), 'Targeting the Leukemia Antigen PR1 with Immunotherapy for the Treatment of Multiple Myeloma', Clin Cancer Res. 24(14): pp 3386-3396. Available at: doi:10.1158/1078-0432.CCR-17-2626.
Sekheri, M., et al., (2020), '15-Epi-LXA4 and 17-epi-RvD1 restore TLR9-mediated impaired neutrophil phagocytosis and accelerate resolution of lung inflammation', PNAS, 117(14): pp 7971–7980. Available at: www.pnas.org/cgi/doi/10.1073/pnas.1920193117
Tacnet-Delorme, P., et al., (2018), ' Proteinase 3 Interferes With C1q-Mediated Clearance of Apoptotic Cells', Front. Immunol. 9: pp 818. Available at: doi: 10.3389/fimmu.2018.00818
NCBI: https://www.ncbi.nlm.nih.gov/protein/P24158/
Shipped with dry ice
Gel Scan of Human Proteinase 3 (PR3)
| Fig. 1: SDS-PAGE Gel |
Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.
To buy Human Proteinase 3 (PR3) please click on the Shopping List Symbol right from the indicated price. This will add Human Proteinase 3 (PR3) to your Shopping List. You can then click on the button »Shopping List & Order Form« to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable.
For business customers only. All prices are net prices (excluding VAT). Final prices in our quote will contain VAT plus shipping costs if applicable. For further information please go to our Shipping page.
Errors, changes and availability excepted.
Payment Methods
Paying by bank transfer
