Human TGF-beta1 (glycosylated, CHO) recombinant Protein

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Product Overview, Sizes and Prices

 

Protein

Synonyms: Transforming growth factor beta-1, TGF-beta-1, TGF-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1.

Source: CHO

Biological activity: ≥ 2 x 107units/mg

Purity: ≥ 95 % (SDS-PAGE)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1935.954.002 2 g on request
1935.954.010 10 g 150 €
1935.954.050 50 g 490 €
1935.954.100 100 g 790 €
1935.954.500 500 g 2.490 €
1935.954.199 1 mg 3.900 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

Transforming growth factor beta-1, TGF-beta-1, TGF-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1.

 

Description of Human TGF-beta1 (glycosylated, CHO)

Recombinant Human Transforming Growth Factor beta-1 (TGF-beta-1) produced in CHO cells is a glycosylated homodimeric polypeptide chain containing 2x112 amino acids with a total molecular mass of 25.6kDa. Transforming Growth Factor-beta-1 (TGF-beta1) is a member of the TGF superfamily (Activin, BMP, GDF, TGF-beta) of homologous, disulfide-linked, homodimer proteins that regulate the proliferation and differentiation of normal and transformed cells. Recombinant human TGF-beta-11 is used for the cultivation of embryonic stem cells and induced pluripotent stem cells. It promotes Th17 and Treg differentiation of T cells. The three mammalian isoforms TGF-beta-1, TGF-beta-2 and TGF-beta-3, signal through the same receptor and elicit similar biological responses. TGF-beta-1 is the most abundant isoform secreted by almost every cell type. All TGF-beta isoforms are involved in various physiological processes, including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. The release of the biologically active TGF-beta isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as Thrombospondin-1 (order number: 4070.952.xyz).

 

Biological Activity

The ED50 of ≤0.05ng/ml was determined by the dose-dependent inhibition of IL-4-induced proliferation of HT-2 cells, it corresponds to a specific activity of ≥2x107 units/mg.

 

Reconstitution

We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. To prepare aliquots we recommend to further dilute into a buffer containing a carrier protein (e.g. 0.1% HSA ultra pure 2835.958.188) and store in working aliquots at -20C to -80C. Working aliquots should be at the highest possible concentration.

 

Stability

Lyophilized TGF-beta 1 is stable at room temperature for up to 3 weeks and at least until the expiry date if it is stored desiccated below -20C. Upon reconstitution TGF-beta-1 can be stored at 4C for 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA, order number: 2835.919.xyz). Please avoid repeated freeze-thaw cycles.

 

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Amino Acid Sequence

ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS

 

Shipping symbol Shipped at room temperature

 

 

 

Biological Activity of Human TGF-beta1 (glycosylated, CHO)

<strong>Fig. 1</strong>: TGF-beta1 dose dependent inhibition of murine IL-4-induced proliferation of mouse HAT-2 cells. Values are the means of triplicate determinations.

Fig. 1: TGF-beta1 dose dependent inhibition of murine IL-4-induced proliferation of mouse HAT-2 cells. Values are the means of triplicate determinations.

 

 

 

 

 

 

 

 

 

To buy Human TGF-beta1 (glycosylated, CHO) (W1935954002, W1935954010, W1935954050, W1935954100, W1935954500, W1935954199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button Shopping List & Order Form or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable.

 

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Errors, changes and availability excepted.

 

 

 

Payment Methods

Paying by bank transfer

Shipping

We ship with UPS

Free shipping for orders above 500 € within Germany

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