Human TGF-beta1 (glycosylated, HEK) recombinant Protein

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Product Overview, Sizes and Prices

 

Protein

Synonyms: Transforming Growth Factor-1, Differentiation inhibiting factor, Cartilage-inducing factor.

Source: HEK

Biological activity: ≥ 2 x 107units/mg

Purity: ≥ 98 % (SDS-PAGE, HPLC)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1935.955.002 2 g on request
1935.955.010 10 g 150 €
1935.955.050 50 g 490 €
1935.955.100 100 g 790 €
1935.955.500 500 g 2.490 €
1935.955.199 1 mg 3.900 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

Transforming Growth Factor-1, Differentiation inhibiting factor, Cartilage-inducing factor.

 

Description of Human TGF-beta1 (glycosylated, HEK)

Recombinant human Transforming Growth Factor-beta 1 (TGF-b1) is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond. The three mammalian isoforms of TGF-beta, TGF-beta1,-beta2, -beta3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodelling and wound healing. They are secreted predominantly as latent complex which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-beta isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-beta1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors.

 

Biological Activity

The ED50 of ≤ 0.05 ng/ml was determined by TGF-beta 1s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. It corresponds to a specific activity of ≥ 2 x 107 units/mg.

 

Reconstitution

We recommend a quick spin followed by reconstitution in sterile Citric Acid (10mM; pH 3.0) to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20C to -80C.

 

Stability

The lyophilized protein is stable at room temperature for up to 1 month, at 4C for up to 6 months, and below -20C until expiry date. Reconstituted protein is stable for at least 12 months when stored in working aliquots with a carrier protein at -20C to -80C. Please avoid repeated freeze-thaw cycles.

 

Lupe Zoom

Amino Acid Sequence

ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS

 

Shipping symbol Shipped at room temperature

 

 

 

 

 

 

 

 

To buy Human TGF-beta1 (glycosylated, HEK) (W1935955002, W1935955010, W1935955050, W1935955100, W1935955500, W1935955199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button Shopping List & Order Form or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable.

 

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Errors, changes and availability excepted.

 

 

 

Payment Methods

Paying by bank transfer

Shipping

We ship with UPS

Free shipping for orders above 500 € within Germany

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