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Human TGF-beta1 (glycosylated, HEK) recombinant Protein
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Protein Synonyms: Transforming Growth Factor-ß1, Differentiation inhibiting factor, Cartilage-inducing factor. Source: HEK Biological activity: ≥ 2 x 107units/mg Purity: ≥ 98 % (SDS-PAGE, HPLC) Physical state: Lyophilized Manufacturer: |
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Active Bioscience
Product Details
Synonyms
Transforming Growth Factor-ß1, Differentiation inhibiting factor, Cartilage-inducing factor.
Description of Human TGF-beta1 (glycosylated, HEK)
Recombinant human Transforming Growth Factor-beta 1 (TGF-b1) is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond. The three mammalian isoforms of TGF-beta, TGF-beta1,-beta2, -beta3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodelling and wound healing. They are secreted predominantly as latent complex which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-beta isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-beta1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors.
Biological Activity
The ED50 of ≤ 0.05 ng/ml was determined by TGF-beta 1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. It corresponds to a specific activity of ≥ 2 x 107 units/mg.
Reconstitution
We recommend a quick spin followed by reconstitution in sterile Citric Acid (10mM; pH 3.0) to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Stability
The lyophilized protein is stable at room temperature for up to 1 month, at 4°C for up to 6 months, and below -20°C until expiry date. Reconstituted protein is stable for at least 12 months when stored in working aliquots with a carrier protein at -20°C to -80°C. Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
Shipped at room temperature
Biological Activity of Human TGF-beta1 (glycosylated, HEK)
| Fig. 1: TGF-beta1 (glycosylated, HEK) Inhibition of Murine IL-4-induced Proliferation in HT2 cells (HT2212-01) |
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Payment Methods
Paying by bank transfer
