Human TGF-beta3 recombinant Protein

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Product Overview, Sizes and Prices

 

Protein

Synonyms: Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.

Source: E. coli

Biological activity: ≥ 2 x 107 units/mg

Purity: ≥ 98 % (SDS-PAGE, HPLC)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1938.950.002 2 g 60 €
1938.950.010 10 g 159 €
1938.950.199 100 g 895 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.

 

Description of Human TGF-beta3

Recombinant human TGF-β3 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond. The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodelling and wound healing. They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-beta isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. The physiological role of TGF-β3 is still unknown but its expression pattern suggests a role in the regulation of certain development processes.

 

Biological Activity

The ED50 of ≤ 0.05 ng/ml was determined by TGF-β3s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. It corresponds to a specific activity of ≥ 2 x 107 units/mg.

 

Reconstitution

We recommend a quick spin followed by reconstitution in 5-10mM Citric Acid to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20C to -80C.

 

Stability

The lyophilized protein is stable at room temperature for up 1 month. Working aliquots stored with a carrier protein are stable for at least 3 months at -20C to -80C. Please avoid repeated freeze thaw cycles.

 

Amino Acid Sequence

MALDTNYCFR NLEENCCVRP LYIDFRQDLG WKWVHEPKGY YANFCSGPCP YLRSADTTHS TVLGLYNTLN PEASASPCCV PQDLEPLTIL YYVGRTPKVE QLSNMVVKSC KCS

 

 

Biological Activity of recombinant Human TGF-beta3 (E. coli)

Fig. 1: Inhibition of mouse IL4-induced proliferation in HT2 cells by Human TGF-beta3. HT2 cells were stimulated with 40ng/ml mouse IL4 and inhibited with 4g/ml Human TGF-beta3. Values are the means (SD) of triplicate determinations and expressed as percentage of control.

Fig. 1: Inhibition of mouse IL4-induced proliferation in HT2 cells by Human TGF-beta3. HT2 cells were stimulated with 40ng/ml mouse IL4 and inhibited with 4g/ml Human TGF-beta3. Values are the means (SD) of triplicate determinations and expressed as percentage of control.

 

 

 

Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.

 

 

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Errors, changes and availability excepted.

 

 

Payment Methods

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Shipping

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Free shipping for orders above 500  within Germany

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