Human TGF-beta3 recombinant Protein

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Product Overview, Sizes and Prices

 

Protein

Synonyms: Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.

Source: E. coli

Biological activity: ≥ 2 x 107 units/mg

Purity: ≥ 98 % (SDS-PAGE, HPLC)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1938.950.002 2 µg 60 €
1938.950.010 10 µg 159 €
1938.950.050 50 µg 495 €
1938.950.100 100 µg 810 €
1938.950.199 1 mg 3.990 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.

 

Description of Human TGF-beta3

Recombinant human TGF-β3 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond. The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodelling and wound healing. They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-beta isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. The physiological role of TGF-β3 is still unknown but its expression pattern suggests a role in the regulation of certain development processes.

 

Biological Activity

The ED50 of ≤ 0.05 ng/ml was determined by TGF-β3’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. It corresponds to a specific activity of ≥ 2 x 107 units/mg.

 

Reconstitution

We recommend a quick spin followed by reconstitution in 5-10mM Citric Acid to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

 

Stability

The lyophilized protein is stable at room temperature for up 1 month. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C. Please avoid repeated freeze thaw cycles.

 

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Amino Acid Sequence

MALDTNYCFR NLEENCCVRP LYIDFRQDLG WKWVHEPKGY YANFCSGPCP YLRSADTTHS TVLGLYNTLN PEASASPCCV PQDLEPLTIL YYVGRTPKVE QLSNMVVKSC KCS

 

Shipping symbol Shipped at room temperature

 

 

 

Biological Activity of recombinant Human TGF-beta3 (E. coli)

<strong>Fig. 1</strong>: Inhibition of mouse IL4-induced proliferation in HT2 cells by Human TGF-beta3. HT2 cells were stimulated with 40ng/ml mouse IL4 and inhibited with 4µg/ml Human TGF-beta3. Values are the means (±SD) of triplicate determinations and expressed as percentage of control.

Fig. 1: Inhibition of mouse IL4-induced proliferation in HT2 cells by Human TGF-beta3. HT2 cells were stimulated with 40ng/ml mouse IL4 and inhibited with 4µg/ml Human TGF-beta3. Values are the means (±SD) of triplicate determinations and expressed as percentage of control.

<strong>Fig. 2</strong>: Inhibition of mouse IL4-induced proliferation by Human TGF-beta1/beta3. Values are the means (±SD) of triplicate determinations and expressed as percentage of control.

Fig. 2: Inhibition of mouse IL4-induced proliferation by Human TGF-beta1/beta3. Values are the means (±SD) of triplicate determinations and expressed as percentage of control.

 

 

 

 

 

 

 

 

 

To buy Human TGF-beta3 (W1938950002, W1938950010, W1938950050, W1938950100, W1938950199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button »Shopping List & Order Form« or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable.

 

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Payment Methods

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Shipping

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