Human TNF-alpha, cct-premium (Yeast) recombinant Protein
Please log in to see the special conditions of your institution.
Phone: +49 (0) 40 . 43 20 84 48 0
Human Tumor Necrosis Factor-alpha, recombinant
Description of Human TNF-alpha, cct-premium (Yeast)
Recombinant human Tumor Necrosis Factor-alpha (TNF-a) is a soluble 17.4 kDa protein containing 157 amino acid residues which corresponds to C-terminal extracellular domain of the full length transmembrane protein. TNF-a is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation and diabetes.
The ED50 of ≤ 0.025 ng/ml was determined after lyophilization in direct comparison to the WHO Standards (NIBSC code 88/786) by a standard cell cytotoxicity assay using the TNF-alpha susceptible mouse cell line L929 in the presence of 1 µg/ml Actinomycin D.1) It corresponds to a specific activity of ≥ 4.0 x 107 IU/mg.
1) Baarsch, M.J. et al. (1991) Detection of tumor necrosis factor alpha from porcine alveolar macrophages using an L929 fibroblast bioassay. J. Immunol. Methods 140:15-22.
We recommend a quick spin followed by reconstitution in sterile-filtered water to a concentration not less than 100 µg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1 % BSA or HSA).
The lyophilized protein is stable at room temperature for a few weeks, but best stored at -20°C. Reconstituted Human TNF-alpha should be used immediately or divided into aliquots and stored at -20°C for future use. For long term storage it is recommended to add a carrier protein (0.1 % HSA or BSA). Please avoid repeated freeze-thaw cycles.
Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.
To buy Human TNF-alpha, cct-premium (Yeast) (W1960953010, W1960953050, W1960953100, W1960953199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button »Shopping List & Order Form« to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable.
All prices are net prices (excluding VAT). Final prices in our quote will contain VAT plus shipping costs if applicable. For further information please go to our Shipping page.
Errors, changes and availability excepted.
Paying by bank transfer
We ship with UPS
Free shipping for orders above 500 € within Germany