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Human TNF-alpha, cct-premium (Yeast) recombinant Protein
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Protein Synonyms: Human Tumor Necrosis Factor-alpha, recombinant. Source: Yeast Biological activity: ≥ 4 x 107 IU/mg Purity: ≥ 97 % (SDS-PAGE) Physical state: Lyophilized Manufacturer: |
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Active Bioscience
Product Details
Synonyms
Human Tumor Necrosis Factor-alpha, recombinant.
Description of Human TNF-alpha, cct-premium (Yeast)
Recombinant human Tumor Necrosis Factor-alpha (TNF-a) is a soluble 17.4 kDa protein containing 157 amino acid residues which corresponds to C-terminal extracellular domain of the full length transmembrane protein. TNF-a is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation and diabetes.
Biological Activity
The ED50 of ≤ 0.025 ng/ml was determined after lyophilization in direct comparison to the WHO Standards (NIBSC code 88/786) by a standard cell cytotoxicity assay using the TNF-alpha susceptible mouse cell line L929 in the presence of 1 µg/ml Actinomycin D.1) It corresponds to a specific activity of ≥ 4.0 x 107 IU/mg.
1) Baarsch, M.J. et al. (1991) Detection of tumor necrosis factor alpha from porcine alveolar macrophages using an L929 fibroblast bioassay. J. Immunol. Methods 140:15-22.
Reconstitution
We recommend a quick spin followed by reconstitution in sterile-filtered water to a concentration not less than 100 µg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1 % HSA - order number: 2835.955.xyz or 2835.958.xyz - or BSA).
Stability
The lyophilized protein is stable at room temperature for a few weeks, but best stored at -20°C. Reconstituted Human TNF-alpha should be used immediately or divided into aliquots and stored at -20°C for future use. For long term storage it is recommended to add a carrier protein (0.1 % HSA or BSA). Please avoid repeated freeze-thaw cycles.
Shipped at room temperature
To buy Human TNF-alpha, cct-premium (Yeast) (W1960953010, W1960953050, W1960953100, W1960953199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button »Shopping List & Order Form« or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable.
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Payment Methods
Paying by bank transfer
