Human TNF-alpha, cct-premium (Yeast) recombinant Protein

Please log in to see the special conditions of your institution.

 

Mitarbeiterfoto

+49 (0) 40 . 43 20 84 48 0

 

Product Overview, Sizes and Prices

 

Protein

Synonyms: Human Tumor Necrosis Factor-alpha, recombinant.

Source: Yeast

Biological activity: ≥ 4 x 107 IU/mg

Purity: ≥ 97 % (SDS-PAGE)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1960.953.010 10 µg 90 €
1960.953.050 50 µg 195 €
1960.953.100 100 µg 375 €
1960.953.199 1 mg 2.750 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

Human Tumor Necrosis Factor-alpha, recombinant.

 

Description of Human TNF-alpha, cct-premium (Yeast)

Recombinant human Tumor Necrosis Factor-alpha (TNF-a) is a soluble 17.4 kDa protein containing 157 amino acid residues which corresponds to C-terminal extracellular domain of the full length transmembrane protein. TNF-a is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation and diabetes.

 

Biological Activity

The ED50 of ≤ 0.025 ng/ml was determined after lyophilization in direct comparison to the WHO Standards (NIBSC code 88/786) by a standard cell cytotoxicity assay using the TNF-alpha susceptible mouse cell line L929 in the presence of 1 µg/ml Actinomycin D.1) It corresponds to a specific activity of ≥ 4.0 x 107 IU/mg.
1) Baarsch, M.J. et al. (1991) Detection of tumor necrosis factor alpha from porcine alveolar macrophages using an L929 fibroblast bioassay. J. Immunol. Methods 140:15-22.

 

Reconstitution

We recommend a quick spin followed by reconstitution in sterile-filtered water to a concentration not less than 100 µg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1 % HSA - order number: 2835.955.xyz or 2835.958.xyz - or BSA).

 

Stability

The lyophilized protein is stable at room temperature for a few weeks, but best stored at -20°C. Reconstituted Human TNF-alpha should be used immediately or divided into aliquots and stored at -20°C for future use. For long term storage it is recommended to add a carrier protein (0.1 % HSA or BSA). Please avoid repeated freeze-thaw cycles.

 

Shipping symbol Shipped at room temperature

 

 

 

 

 

 

 

 

To buy Human TNF-alpha, cct-premium (Yeast) (W1960953010, W1960953050, W1960953100, W1960953199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button »Shopping List & Order Form« or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable.

 

All prices are net prices (excluding VAT). Final prices in our quote will contain VAT plus shipping costs if applicable. For further information please go to our Shipping page.

 

Errors, changes and availability excepted.

 

 

 

Payment Methods

Paying by bank transfer

Shipping

We ship with UPS

Free shipping for orders above 500 € within Germany

This website uses cookies to store your Personal Shopping List. By using our website you accept the use of session cookies. Learn more
I accept all necessary cookies