Murine EGF recombinant Protein
Murine Epidermal Growth Factor, Mouse Epidermal Growth Factor, Urogastrone, URG, EGF.
Description of Murine EGF
Recombinant murine Epidermal Growth Factor (mEGF) derived from E. coli is a 6.0 kDA protein with 3 intramole-cular disulfide-bonds containing 53 amino acids. EGF is a potent growth factor, which stimulates the proliferation of various epidermal and epithelial cells and some fibroblasts. Additionally, EGF has been shown to inhibit gastric secre-tion, and to be involved in wound healing. EGF signals trough a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-alpha and VGF (vaccinia virus growth factor). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation and colony formation of epidermal cells in culture.
The ED50 of ≤ 0.1ng/ml was determined by the dose dependent stimulation of the proliferation of murine BALB/c 3T3 cells. It corresponds to a specific activity of ≥ 1 x 107 units/mg.
We recommend a quick spin followed by reconstitution in sterile water to a concentration of at least 100µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For long term storage, it is recommended to further dilute in a buffer - if possible - containing a carrier (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA, order number: 2835.919.xyz) and store in working aliquots at -20°C to -80°C.
The lyophilized protein is stable at room temperature for up to 1 month and below -20°C until expiry date. Reconstituted protein is stable for at least 12 months when stored in working aliquots with a carrier at -20°C to -80°C. Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR
Shipped at room temperature
To buy Murine EGF (W1325960100, W1325960500, W1325960199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button »Shopping List & Order Form« or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable. Murine EGF is an alternative for the no longer available Promokine EGF, mouse, recombinant (D-60170).
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