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Murine IFN-gamma recombinant Protein
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Protein Synonyms: Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma. Source: E. coli Biological activity: ≥ 1.0 x 107 units/mg Purity: ≥ 95 % (SDS-PAGE, RP-HPLC) Physical state: Lyophilized Manufacturer: |
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Active Bioscience
Product Details
Synonyms
Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.
Description of Murine IFN-gamma
Recombinant Murine Interferon-gamma is a 15.6 kDa protein containing 134 amino acid residues. IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-gamma signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-gamma stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-gamma species-specific and is biologically active only in human and primate cells.
Biological Activity
The ED50 of ≤ 0.1ng/ml was determined in a viral resistance assay (L-292 mouse fibroblast infected with EMC). It corresponds to a specific activity of ≥ 1.0 x 107 units/mg.
Reconstitution
We recommend a quick spin followed by reconstitution in sterile filtered water or 20mM acetic acid to a concentration of 0.1 - 0.5 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA or Trehalose) and store in working aliquots at -20°C to -80°C.
Stability
Lyophilized IFN-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-gamma should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA, order number: 2835.919.xyz). Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC
Shipped at room temperature
To buy Murine IFN-gamma (W1476960020, W1476960100, W1476960199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button »Shopping List & Order Form« or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable. Murine IFN-gamma is an alternative for the no longer available Promokine IFN-gamma, mouse, recombinant (D-60724).
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Errors, changes and availability excepted.
Payment Methods
Paying by bank transfer
