Murine IL-1 alpha recombinant Protein
Product Details
Synonyms
Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha,IL1, IL-1A, IL1F1.
Description of Murine IL-1 alpha
Recombinant murine Interleukin-1alpha (IL-1alpha), produced in E.coli is a 17.9 kDa protein with 156 amino acid residues. It is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is a predominantly a cell-associated cytokine.
Biological Activity
The ED50 of ≤ 0.002 ng/ml was determined by the dose-dependent stimulation of the proliferation of murine D10S cells. It corresponds to a specific activity of ≥ 5.0 x 108 units/mg.
Reconstitution
We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20°C for future use.
Stability
The lyophilized protein is stable at room temperature for a few weeks, but best stored at 20°C. Reconstituted murine IL-1 alpha should be stored in working aliquots at 20°C. For Long term storage we recommend the addition of a carrier Protein e.g. 0,1% highly pure HSA (order number: 2835.958.xyz) or BSA (order number: 2835.919.xyz). Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS
Shipped at room temperature
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Payment Methods
Paying by bank transfer
