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Murine IL-1 alpha recombinant Protein
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Protein Synonyms: Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha,IL1, IL-1A, IL1F1. Source: E. coli Biological activity: ≥ 5.0 x 108 units/mg Purity: ≥ 97 % (SDS-PAGE, HPLC) Physical state: Lyophilized Manufacturer: |
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Active Bioscience
Product Details
Synonyms
Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha,IL1, IL-1A, IL1F1.
Description of Murine IL-1 alpha
Recombinant murine Interleukin-1alpha (IL-1alpha), produced in E.coli is a 17.9 kDa protein with 156 amino acid residues. It is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is a predominantly a cell-associated cytokine.
Biological Activity
The ED50 of ≤ 0.002 ng/ml was determined by the dose-dependent stimulation of the proliferation of murine D10S cells. It corresponds to a specific activity of ≥ 5.0 x 108 units/mg.
Reconstitution
We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20°C for future use.
Stability
The lyophilized protein is stable at room temperature for a few weeks, but best stored at 20°C. Reconstituted murine IL-1 alpha should be stored in working aliquots at 20°C. For Long term storage we recommend the addition of a carrier Protein e.g. 0,1% highly pure HSA (order number: 2835.958.xyz) or BSA (order number: 2835.919.xyz). Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS
Shipped at room temperature
To buy Murine IL-1 alpha please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button »Shopping List & Order Form« or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable. Murine IL-1 alpha is an alternative for the no longer available Promokine IL-1 alpha, mouse, recombinant (D-61112).
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Errors, changes and availability excepted.
Payment Methods
Paying by bank transfer
