Murine IL-10 recombinant Protein

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Product Overview, Sizes and Prices
	Proteins Synonyms: B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor. Biological Activity: ≥ 1 x 106 units/mg Source: E. coli Purity: ≥ 97 % (SDS-PAGE, RP-HPLC) Endotoxin Level: ≤ 0.1ng/µg ( ≤ 1EU/µg) Buffer: PBS (pH 7.4) Physical State: Lyophilized Manufacturer:  Active Bioscience	Article No. Size Price   1541.960.002 2 µg 90 € 1541.960.010 10 µg 175 € 1541.960.199 1 mg 3.990 € Please inquire for other vial sizes and custom vialing.

 

 

Produktdetails

Synonyms

B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.

 

Description of Murine IL-10

Recombinant Murine Interleukin-10 (mIL-10) derived from E. coli is a single, non-glycosylated protein consisting of 161 amino acids with a molecular mass of 18.8 kDa. Interleukin-10 (IL-10) is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

 

Physcial State

Sterile filtered, lyophilized

 

Biological Activity

The ED₅₀ of ≤ 1ng/ml was determined by a cell proliferation assay using murine MC/9-2 cells. This corresponds to a specific activity of ≥ 1 x 10⁶ units/mg.

 

Reconstitution

We recommend a quick spin followed by reconstitution in sterile 10mM HCl to a concentration of at least 100 µg/ml, which can then be further diluted to other aqueous solutions. Do not vortex.

 

Stability

Lyophilized murine IL-10 is stable at room temperature for up to 3 weeks, and until expiry date if stored below 18°C. Upon reconstitution murine Il-10 can be stored at 4°C for 2-7 days and for future use below 18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA, order number: 2835.919.xyz). Please avoid repeated freeze-thaw cycles.

 

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Amino Acid Sequence

SRGQYSREDN NCTHFPVGQS HMLLELRTAF SQVKTFFQTK DQLDNILLTD SLMQDFKGYL GCQALSEMIQ FYLVEVMPQA EKHGPEIKEH LNSLGEKLKT LRMRLRRCHR FLPCENKSKA VEQVKSDFNK LQDQGVYKAM NEFDIFINCI EAYMMIKMKS

 

Shipped at room temperature

 

 

Download Datasheet

 

 

 

 

 

Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.

 

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