Murine IL-12 p70 (glycosylated, HEK) recombinant Protein
NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.
Description of Murine IL-12 p70 (glycosylated, HEK)
Interleukin-12 Murine Recombinant produced in HEK 293 cells is a glycosylated disulfide linked heterodimeric polypeptide containing 506 amino acids and having a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.
The ED 50 of 10-20 ng/ml was determined by the dose-dependent induced expression of CD25 on CD8+ mouse T cells, it corresponds to a specific activity of 5-10 x 104 units/mg.
It is recommended to reconstitute the lyophilized murine IL12 in sterile distilled pyrogen free water at 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized mouse IL-12 although stable at room temperature for 3 weeks, should be stored below -18°C. Upon reconstitution IL12 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA, order number: 2835.919.xyz). Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
p35Subunit :RVIPVSGPA RCLSQSRNLL KTTDDMVKTA REKLKHYSCT AEDIDHEDIT RDQTSTLKTC LPLELHKNES CLATRETSST TRGSCLPPQK TSLMMTLCLG SIYEDLKMYQ TEFQAINAAL QNHNHQQIIL DKGMLVAIDE LMQSLNHNGE TLRQKPPVGE ADPYRVKMKL CILLHAFSTR VVTINRVMGY LSSA.p40Su bunit:MWEL EKDVYVVEVD WTPDAPGETV NLTCDTPEED DITWTSDQRH GVIGSGKTLT ITVKEFLDAG QYTCHKGGET LSHSHLLLHK KENGIWSTEI LKNFKNKTFL KCEAPNYSGR FTCSWLVQRN MDLKFNIKSS SSSPDSRAVT CGMASLSAEK VTLDQRDYEK YSVSCQEDVT CPTAEETLPI ELALEARQQN KYENYSTSFF IRDIIKPDPP KNLQMKPLKN SQVEVSWEYP DSWSTPHSYF SLKFFVRIQR KKEKMKETEE GCNQKGAFLV EKTSTEVQCK GGNVCVQAQD RYYNSSCSKW ACVPCRVRS
Shipped at room temperature
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