Murine IL-6 recombinant Protein
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IFN-b2, BSF-2, HPGF, HSF, MGI-2, IL-6, Interleukin HP-1, B cell differentiation factor (BCDF), B-cell hybridoma growth factor.
Description of Murine IL-6
Recombinant murine Interleukin-6 (IL-6) is a 21.7 kDa protein containing 188 amino acid residues. IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimu-lates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system, that consists of two chains IL-6R alpha and gp 130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells.
The ED50 of ≤ 0.02 ng/ml was determined by the dose-dependent stimulation of the proliferation of the IL-6 depend-ent murine 7TD1 cells. It corresponds to a specific activity of ≥ 5 x 107 units/mg.
We recommend a quick spin followed by reconstitution in sterile filtered water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1 % BSA or HSA) and store in working aliquots at -20°C to -80°C.
Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term stor-age it is recommended to add a carrier protein (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA, order number: 2835.919.xyz). Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI ORNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT
Shipped at room temperature
SDS-PAGE and Biological Activity of Murine IL-6
Fig. 1: SDS-PAGE analysis of recombinant Murine IL-6. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie blue.
Fig. 2: Murine IL-6 induced proliferation of mouse hybridoma cell line B9. The cells were stimulated using recombinant Murine IL-6. Values are the means (±SD) of triplicate determinations and expressed as percentage of control.
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