Murine IL-6 recombinant Protein

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Product Overview, Sizes and Prices

 

Protein

Synonyms: IFN-b2, BSF-2, HPGF, HSF, MGI-2, IL-6, Interleukin HP-1, B cell differentiation factor (BCDF), B-cell hybridoma growth factor.

Source: E. coli

Biological activity: ≥ 5 x 107 units/mg

Purity: ≥ 98% (SDS-PAGE)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1525.960.005 5 g 60 €
1525.960.020 20 g 159 €
1525.960.050 50 g 270 €
1525.960.100 100 g 470 €
1525.960.500 500 g 1.390 €
1525.960.199 1 mg 2.390 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

IFN-b2, BSF-2, HPGF, HSF, MGI-2, IL-6, Interleukin HP-1, B cell differentiation factor (BCDF), B-cell hybridoma growth factor.

 

Description of Murine IL-6

Recombinant murine Interleukin-6 (IL-6) is a 21.7 kDa protein containing 188 amino acid residues. IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimu-lates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system, that consists of two chains IL-6R alpha and gp 130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells.

 

Biological Activity

The ED50 of ≤ 0.02 ng/ml was determined by the dose-dependent stimulation of the proliferation of the IL-6 depend-ent murine 7TD1 cells. It corresponds to a specific activity of ≥ 5 x 107 units/mg.

 

Reconstitution

We recommend a quick spin followed by reconstitution in sterile filtered water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1 % BSA or HSA) and store in working aliquots at -20C to -80C.

 

Stability

Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL6 should be stored at 4C between 2-7 days and for future use below -18C.For long term stor-age it is recommended to add a carrier protein (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA, order number: 2835.919.xyz). Please avoid repeated freeze-thaw cycles.

 

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Amino Acid Sequence

MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI ORNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT

 

Shipping symbol Shipped at room temperature

 

 

 

SDS-PAGE and Biological Activity of Murine IL-6

<strong>Fig. 1</strong>: SDS-PAGE analysis of recombinant Murine IL-6. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie blue.

Fig. 1: SDS-PAGE analysis of recombinant Murine IL-6. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie blue.

<strong>Fig. 2</strong>: Murine IL-6 induced proliferation of mouse hybridoma cell line B9. The cells were stimulated using recombinant Murine IL-6. Values are the means (SD) of triplicate determinations and expressed as percentage of control.

Fig. 2: Murine IL-6 induced proliferation of mouse hybridoma cell line B9. The cells were stimulated using recombinant Murine IL-6. Values are the means (SD) of triplicate determinations and expressed as percentage of control.

 

 

 

 

 

 

 

 

 

To buy Murine IL-6 (W1525960005, W1525960020, W1525960050, W1525960100, W1525960500, W1525960199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button Shopping List & Order Form or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable.

 

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Shipping

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