Murine Leptin Triple Antagonist recombinant Protein
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Description of Murine Leptin Triple Antagonist
Leptin Antagonist Triple Mutant Murine Recombinant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of " 16 kDa, LEP was mutated, resulting in L39A/D40A/F41A mutant.
Leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human Leptin receptor can be inhibited by recombinant murine Leptin Antagonist Triple Mutant. It also inhibits various Leptin effects in several in vitro bioassays.
It is recommended to reconstitute the lyophilized Leptin Antagonist Triple Mutant Mouse Recombinant in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted with other aqueous solutions.
Lyophilized Leptin Antagonist Triple Mutant Mouse Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 Leptin mutant mg/ml and up to 2 mM and filter sterilization LEP mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln.
Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.
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