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Murine Sonic Hedgehog / Shh recombinant Protein
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Protein Synonyms: SHH, HHG-1, HHG1, Sonic hedgehog protein. Source: E. coli Purity: ≥ 97 % (SDS-PAGE) Physical state: Lyophilized Manufacturer: |
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Active Bioscience
Product Details
Synonyms
SHH, HHG-1, HHG1, Sonic hedgehog protein.
Description of Murine Sonic Hedgehog / Shh
Sonic Hedgehog Recombinant Murine produced in E. coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 19.8 kDa. The Murine Sonic Hedgehog is 99% homologous to the human gene.Cysteine at position 25 has been substituted with Ile.
Biological Activity
Determined by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 (CCL-226) fibroblasts and is typically 0.48-0.72 µg/ml.
Reconstitution
It is recommended to reconstitute the lyophilized Sonic Hedgehog in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Mouse Sonic HedgeHog although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Sonic HedgeHog should be stored at 4°C between 2-7 days and for future use below -18°C. Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
Shipped at room temperature
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Payment Methods
Paying by bank transfer
