Murine Sonic Hedgehog / Shh recombinant Protein

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Phone: +49 (0) 40 . 43 20 84 48 0

E-Mail: info@active-bioscience.de

 

Product Overview, Sizes and Prices

 

Protein

Synonyms: SHH, HHG-1, HHG1, Sonic hedgehog protein.

Source: E. coli

Purity: ≥ 97 % (SDS-PAGE)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1591.960.005 5 µg 60 €
1591.960.025 25 µg 159 €
1591.960.199 1 mg 2.145 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

SHH, HHG-1, HHG1, Sonic hedgehog protein.

 

Description of Murine Sonic Hedgehog / Shh

Sonic Hedgehog Recombinant Mouse produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 19.8 kDa. The Mouse Sonic Hedgehog is 99% homologous to the human gene.Cysteine at position 25 has been substituted with Ile.The Sonic HedgeHog is purified by proprietary chromatographic techniques.

 

Biological Activity

Determined by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 (CCL-226) fibroblasts and is typically 0.48-0.72 µg/ml.

 

Reconstitution

It is recommended to reconstitute the lyophilized Sonic Hedgehog in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 

Stability

Lyophilized Mouse Sonic HedgeHog although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Sonic HedgeHog should be stored at 4°C between 2-7 days and for future use below -18°C. Please avoid repeated freeze-thaw cycles.

 

Amino Acid Sequence

MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

 

 

 

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Errors, changes and availability excepted.

 

 

Payment Methods

Paying by bank transfer

Shipping

We ship with UPS

Free shipping for orders above 500 € within Germany

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