Rat EGF recombinant Protein

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Product Overview, Sizes and Prices

 

Protein

Synonyms: Rat Epidermal Growth Factor, Urogastrone, URG, EGF.

Source: E. coli

Biological activity: ≥ 1 x 107 units/mg

Purity: ≥ 98 % (SDS-PAGE, RP-HPLC)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1325.970.020 20 g 60 €
1325.970.100 100 g 159 €
1325.970.199 1 mg 890 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

Rat Epidermal Growth Factor, Urogastrone, URG, EGF.

 

Description of Rat EGF

Recombinant rat Epidermal Growth Factor (rEGF) derived from E. Coli is a 6.1 kDA protein containing 53 amino acids. EGF is a potent growth factor, which stimulates the proliferation of various epidermal and epithelial cells and some fibroblasts. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals trough a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-alpha and VGF (vaccinia virus growth factor). Biological activities ascribed to EGF include epithelial devel-opment, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation and colony formation of epidermal cells in culture.

 

Biological Activity

The ED50 of ≤ 0.1ng/ml was determined by the dose dependent stimulation of the proliferation of murine BALB/c 3T3 cells. It corresponds to a specific activity of ≥ 1 x 107 units/mg.

 

Reconstitution

We recommend a quick spin followed by reconstitution in sterile water to a concentration of at least 100g/ml. Do not vortex. This solution can be stored at 2-8C for up to 1 week. For long term storage, it is recommended to further dilute in a buffer - if possible - containing a carrier (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA, order number: 2835.919.xyz) and store in working aliquots at -20C to -80C.

 

Stability

The lyophilized protein is stable at room temperature for up to 1 month and below -20C until expiry date. Reconstituted protein is stable for at least 12 months when stored in working aliquots with a carrier at -20C to -80C. Please avoid repeated freeze-thaw cycles.

 

Lupe Zoom

Amino Acid Sequence

NSNTGCPPSY DGYCLNGGVC MYVESVDRYV CNCVIGYIGE RCQHRDLRWW KLR

 

Shipping symbol Shipped at room temperature

 

 

 

 

 

 

 

 

To buy Rat EGF (W1325970020, W1325970100, W1325970199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button Shopping List & Order Form or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable. Rat EGF is an alternative for the no longer available Promokine EGF, rat, recombinant (E-60170).

 

All prices are net prices (excluding VAT). Final prices in our quote will contain VAT plus shipping costs if applicable. For further information please go to our Shipping page.

 

Errors, changes and availability excepted.

 

 

 

Payment Methods

Paying by bank transfer

Shipping

We ship with UPS

Free shipping for orders above 500 € within Germany

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