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Rat IFN-gamma recombinant Protein
Product Overview, Sizes and Prices |
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Protein Synonyms: Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma. Source: E. coli Biological activity: ≥ 1 x 107 units/mg Purity: ≥ 95 % (SDS-PAGE, RP-HPLC) Physical state: Lyophilized Manufacturer: |
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Active Bioscience
Product Details
Synonyms
Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.
Description of Rat IFN-gamma
Recombinant Rat Interferon-gamma (IFN-gamma) is a 17 kDa protein containing 144 amino acid residues. IFN-gamma is a lymphoid factor which possesses potent anti-viral activity. It also has been shown to stimulate macrophages and NK-cells.
Biological Activity
The ED50 of 0.02-0.04µg/ml was determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The ED50 as determined in a viral resistance assay is 0.05ng/ml. It corresponds to a specific activity of 2.0 x 107 IU/mg. For most in vitro applications, IFN-gamma exerts its biological activity in the concentration range of 0.1 to 10.0ng/ml. Please perform appropriate test to determine the optimal concentration for your application.
Reconstitution
We recommend a quick spin followed by reconstitution water to a concentration of at least 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week or in working aliquots at -20°C to -80°C. Working aliquots should be at the highest practical concentration. For long term storage we recommend adding at least 0.1% HSA (order number: 2835.955.xyz or 2835.958.xyz) or BSA (order number: 2835.919.xyz).
Stability
The lyophilized protein is stable at room temperature for a few weeks, but best stored desiccated below -20°C. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. Please avoid repeated freeze thaw cycles.
Amino Acid Sequence
MQGTLIESLE SLKNYFNSSS MDAMEGKSLL LDIWRNWQKD GNTKILESQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT NFFSNSKAKK DAFMSIAKFE VNNPQIQHKA VNELIRVIHQ LSPESSLRKR KRSRC
Shipped at room temperature
To buy Rat IFN-gamma (W1476970020, W1476970100, W1476970199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button »Shopping List & Order Form« or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable. Rat IFN-gamma is an alternative for the no longer available Promokine IFN-gamma, rat, recombinant (E-60730).
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Errors, changes and availability excepted.
Payment Methods
Paying by bank transfer
