Human C3c

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Synonyms

Complement C3c, Complement Component C3c, C3c.

Description of Human C3c

Human Complement component C3c (hC3c) derived from human plasma consist of 3 peptides with a total molecular mass of 138 KDa. The three peptides (C3c Beta chain (23-667), C3c alpha chain fragment 1 (749-954) and C3c alpha chain fragment 2 (1321-1663)) are joined together by disulphide bonds. hC3c plays an important role in both complement activation pathways, with different specific proteolytic systems cleaving it to form C3 convertase. Cleavage of C3 releases C3a and the C3b fragment which is part of the alternative C3 convertase. C3 levels can be low because of decreased synthesis or due to consumption. High C3 levels are seen in highly acute or chronic inflammation, hepatic cholestasis and during the third trimester of pregnancy. Unwanted complement activation is a major cause of tissue damage in various pathological conditions and contributes to quite a few immune complex diseases.

Reconstitution

We recommend a quick spin followed by reconstitution in sterile water to a concentration of 1-2mg/ml. Mix thoroughly and centrifuge briefly to ensure complete recovery. Do not vortex! Further dilutions should be prepared with PBS.

Stability

Lyophilized human Human C3c although stable at 2-8°C for 3 weeks, should be stored desiccated at -20°C, Reconstituted human hC3c should be stored at 4°C between 2-7 days and in working aliquots below -18°C for future use. Please avoid repeated freeze-thaw cycles.

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Amino Acid Sequence

C3c betachain (23-667)
SPMYSIITPN ILRLESEETM VLEAHDAQGD VPVTVTVHDF PGKKLVLSSE KTVLTPATNH MGNVTFTIPA NREFKSEKGR NKFVTVQATF GTQVVEKVVL VSLQSGYLFI QTDKTIYTPG STVLYRIFTV NHKLLPVGRT VMVNIENPEG IPVKQDSLSS QNQLGVLPLS WDIPELVNMG QWKIRAYYEN SPQQVFSTEF EVKEYVLPSF EVIVEPTEKF YYIYNEKGLE VTITARFLYG KKVEGTAFVI FGIQDGEQRI SLPESLKRIP IEDGSGEVVL SRKVLLDGVQ NPRAEDLVGK SLYVSATVIL HSGSDMVQAE RSGIPIVTSP YQIHFTKTPK YFKPGMPFDL MVFVTNPDGS PAYRVPVAVQ GEDTVQSLTQ GDGVAKLSIN THPSQKPLSI TVRTKKQELS EAEQATRTMQ ALPYSTVGNS NNYLHLSVLR TELRPGETLN VNFLLRMDRA HEAKIRYYTY LIMNKGRLLK

C3c alphachain fragment 1 (749-954)
SNLDEDIIA EENIVSRSEF PESWLWNVED LKEPPKNGIS TKLMNIFLKD SITTWEILAV SMSDKKGICV ADPFEVTVMQ DFFIDLRLPY SVVRNEQVEI RAVLYNYRQN QELKVRVELL HNPAFCSLAT TKRRHQQTVT IPPKSSLSVP YVIVPLKTGL QEVEVKAAVY HHFISDGVRK SLKVVPEGIR MNKTVAVRTL DPERLGR

C3c alphachain fragment 2 (1321-1663)
SEETKENEGF TVTAEGKGQG TLSVVTMYHA KAKDQLTCNK FDLKVTIKPA PETEKRPQDA KNTMILEICT RYRGDQDATM SILDISMMTG FAPDTDDLKQ LANGVDRYIS KYELDKAFSD RNTLIIYLDK VSHSEDDCLA FKVHQYFNVE LIQPGAVKVY AYYNLEESCT RFYHPEKEDG KLNKLCRDEL CRCAEENCFI QKSDDKVTLE ERLDKACEPG VDYVYKTRLV KVQLSNDFDE YIMAIEQTIK SGSDEVQVGQ QRTFISPIKC REALKLEEKK HYLMWGLSSD FWGEKPNLSY IIGKDTWVEH WPEEDECQDE ENQKQCQDLG AFTESMVVFG CPN

Shipped at room temperature

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