Human FGF-23 recombinant Protein

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Synonyms

Fibroblast Growth Factor-23, FGF-23, FGF23, Tumor-derived hypophosphatemia-inducing factor, HYPF, ADHR, HPDR2, PHPTC.

Description of Human FGF-23

Recombinant Human Fibroblast Growth Factor-23 (FGF-23) is a 228 amino acid protein, with a molecular mass of 22,5 kDa. It is a heparin-binding growth factor belonging to the fibroblast growth factor (FGF) family. FGF family members possess extensive mitogenic and cell survival functions, and are involved in various biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Together with FGF-21 and FGF-19 it constitutes an atypical FGF subfamily whose ligands act as circulating hormones and require the participation of a Klotho protein (vergl.1628.954.xyz) as a co-receptor for their signaling. FGF-23 is a bone-derived hormone that acts in the kidney to regulate phosphate homeostasis and vitamin D metabolism. The signaling receptor for FGF-23, a Klotho-FGFR1 (IIIc) complex, is an essential regulator of the renal sodium phosphate co-transporter and key vitamin D-metabolizing enzymes CYP27B1 and CYP24A1.

Biological Activity

The ED₅₀ of ≤ 0.05-0.5 µg/ml was determined by the dose-dependent proliferation of NIH/3T3 in the presence of recombinant murine Klotho (5µg/ml) and heparin (10µg/ml).

Reconstitution

We recommend a quick spin followed by reconstitution in water to a concentration of at least 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week or in working aliquots at -20°C to -80°C. Working aliquots should be at the highest practical concentration. For long term storage we recommend to add at least 0.1% HSA (order number: 2835.955.xyz or 2835.958.xyz) or BSA (order number: 2835.919.xyz).

Stability

The lyophilized protein is stable at room temperature for up to 1 month and at least until the lot specific expiry date if kept below -18°C. Reconstituted FGF-23 should be stored in working aliquots at -20°C to -80°C if possible with carrier protein. Please avoid repeated freeze thaw cycles.

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Amino Acid Sequence

MYPNASPLLG SSWGGLIHLY TATARNSYHL QIHKNGHVDG APHQTIYSAL MIRSEDAGFV VITGVMSRRY LCMDFRGNIF GSHYFDPENC RFQHQTLENG YDVYHSPQYH FLVSLGRAKR AFLPGMNPPP YSQFLSRRNE IPLIHFNTPI PRRHTRSAED DSERDPLNVL KPRARMTPAP ASCSQELPSA EDNSPMASDP LGVVRGGRVN THAGGTGPEG CRPFAKFI

Shipped at room temperature

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