Human FSH (glycosylated) recombinant Protein

Synonyms

Follitropin subunit beta, Follicle-stimulating hormone beta subunit, FSH-beta, FSH-B, Follitropin beta chain, FSH.

Description of Human FSH (glycosylated)

FSH Human Recombinant produced in HEK-293 cells is heterodimeric, glycosylated, polypeptide chain transfected with two expression plasmids encoding the human FSH-alpha chain (Accession # P01215) (Ala25-Ser116) and human FSH-beta chain (Asn19-Glu129) (Accession # P01225) having a total Mw of 38kDa. FSH human recombinant is purified by proprietary chromatographic techniques.

Biological Activity

The ED₅₀ as determined by cAMP accumulation in human FSH Receptor transfected Chinese Hamster Ovary cells was found to be 80-450 pg/ml.

Reconstitution

It is recommended to reconstitute the lyophilized Follicle Stimulating Hormone in sterile 18MΩ-cm H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized recombinant FSH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FSH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.

Amino Acid Sequence

FSHsubunit alpha:APDV QDCPECTLQE NPFFSQPGAP ILQCMGCCFS RAYPTPLRSK KTMLVQKNVT SESTCCVAKS YNRVTVMGGF KVENHTACHC STCYYHKS.F SHsubunitb eta:NSCELT NITIAIEKEE CRFCISINTT WCAGYCYTRD LVYKDPARPK IQKTCTFKEL VYETVRVPGC AHHADSLYTY PVATQCHCGK CDSDSTDCTV RGLGPSYCSF GEMKE