Human FSH (glycosylated) recombinant Protein
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Follitropin subunit beta, Follicle-stimulating hormone beta subunit, FSH-beta, FSH-B, Follitropin beta chain, FSH.
Description of Human FSH (glycosylated)
FSH Human Recombinant produced in HEK-293 cells is heterodimeric, glycosylated, polypeptide chain transfected with two expression plasmids encoding the human FSH-alpha chain (Accession # P01215) (Ala25-Ser116) and human FSH-beta chain (Asn19-Glu129) (Accession # P01225) having a total Mw of 38kDa. FSH human recombinant is purified by proprietary chromatographic techniques.
The ED50 as determined by cAMP accumulation in human FSH Receptor transfected Chinese Hamster Ovary cells was found to be 80-450 pg/ml.
It is recommended to reconstitute the lyophilized Follicle Stimulating Hormone in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized recombinant FSH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FSH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
FSHsubunit alpha:APDV QDCPECTLQE NPFFSQPGAP ILQCMGCCFS RAYPTPLRSK KTMLVQKNVT SESTCCVAKS YNRVTVMGGF KVENHTACHC STCYYHKS.F SHsubunitb eta:NSCELT NITIAIEKEE CRFCISINTT WCAGYCYTRD LVYKDPARPK IQKTCTFKEL VYETVRVPGC AHHADSLYTY PVATQCHCGK CDSDSTDCTV RGLGPSYCSF GEMKE
Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.
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