Human IFN-alpha 2a recombinant Protein

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Synonyms

Leukocyte IFN, B cell IFN, Type I IFN, IFNA2, IFN-a 2a.

Description of Human IFN-alpha 2a

Recombinant Human Interferon alpha 2a (hIFN alpha 2a) is a single, non-glycosylated, protein consisting of 165 amino acids with a molecular mass of 19.2 kDa. The difference between IFNA2A and IFNA2B is in the amino acid present at position 23. IFN-alpha 2a has a lysine at that position 23 while IFN-alpha 2b has arginine. The IFNA2A gene was obtained from human leukocytes.

Biological Activity

The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 270,000,000 IU/mg.

Reconstitution

We recommend a quick spin followed by reconstitution in sterile water to a concentration of at least 100 µg/ml, which can then be further diluted to other aqueous solutions. Do not vortex.

Stability

Lyophilized IFN-alpha 2a is stable at room temperature for up to 3 weeks, but best stored desiccated below -18°C. Upon reconstitution IFN alpha 2a can be stored at 4°C for 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA, order number: 2835.919.xyz). Please avoid repeated freeze-thaw cycles.

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Amino Acid Sequence

CDLPQTHSLG SRRTLMLLAQ MRKISLFSCL KDRHDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE.
(N-terminal methionine has been completely removed enzymatically)

Shipped at room temperature

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