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Human IFN-alpha 2a recombinant Protein
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Protein Synonyms: Leukocyte IFN, B cell IFN, Type I IFN, IFNA2, IFN-a 2a. Source: E. coli Biological activity: ≥ 2.7 x 108 units/mg Purity: ≥ 97 % (SDS-PAGE, RP-HPLC) Physical state: Lyophilized Manufacturer: |
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Active Bioscience
Product Details
Synonyms
Leukocyte IFN, B cell IFN, Type I IFN, IFNA2, IFN-a 2a.
Description of Human IFN-alpha 2a
Recombinant Human Interferon alpha 2a (hIFN alpha 2a) is a single, non-glycosylated, protein consisting of 165 amino acids with a molecular mass of 19.2 kDa. The difference between IFNA2A and IFNA2B is in the amino acid present at position 23. IFN-alpha 2a has a lysine at that position 23 while IFN-alpha 2b has arginine. The IFNA2A gene was obtained from human leukocytes.
Biological Activity
The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 270,000,000 IU/mg.
Reconstitution
We recommend a quick spin followed by reconstitution in sterile water to a concentration of at least 100 µg/ml, which can then be further diluted to other aqueous solutions. Do not vortex.
Stability
Lyophilized IFN-alpha 2a is stable at room temperature for up to 3 weeks, but best stored desiccated below -18°C. Upon reconstitution IFN alpha 2a can be stored at 4°C for 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA, order number: 2835.919.xyz). Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
CDLPQTHSLG SRRTLMLLAQ MRKISLFSCL KDRHDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE.
(N-terminal methionine has been completely removed enzymatically)
Shipped at room temperature
To buy Human IFN-alpha 2a please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button »Shopping List & Order Form« or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable. Human IFN-alpha 2a is an alternative for the no longer available Promokine IFN-alpha 2a, human, recombinant (E. coli) (C-60580).
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Errors, changes and availability excepted.
Payment Methods
Paying by bank transfer
