Human IFN-beta 1a (glycosylated, HSA) recombinant Protein

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Synonyms

Leukocyte interferon, B cell interferon, Type I interferon, Leukocyte IFN, B cell IFN, Type I IFN, IFNB1, IFB, IFF, IFNB, IFN-b 1a, MGC96956.

Description of Human IFN-beta 1a (glycosylated, HSA)

Recombinant human Interferon-beta 1a (IFN-beta 1a) derived from CHO is a 20.0 kDa protein containing 166 amino acid residues. Due to glycosylation, IFN-beta 1a has an approximate MW of 22.3 kDa based on SDS-PAGE gel and Mass Spectrometry. Proteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells, IFN- alpha is an acid stable molecule that signals through IFN-alpha/-betaR, which is also used by IFN-beta. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. IFN-alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity.

Biological Activity

The biological activity of human IFN-beta was based on analysis using the WISH cell line with VSV as the challenge virus. The specific activity was determined to be ≥ 2.0 x 10⁸ IU/mg.

Reconstitution

We recommend a quick spin followed by reconstitution in sterile water to a concentration of at least 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

The lyophilized protein is stable until the indicated expiry date if stored ≤ -20°C. Reconstituted IFN-beta 1a is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. For long term storage we rec-ommend to add at least 0.1% HSA (order number: 2835.955.xyz or 2835.958.xyz) or BSA (order number: 2835.919.xyz). Please avoid repeated freeze-thaw cycles.

Shipped at room temperature

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