Human IFN-beta 1b recombinant Protein

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Synonyms

Leukocyte IFN, B cell IFN, Type I IFN, IFNB1, IFB, IFF, IFNB, IFN-b 1b, MGC96956.

Description of Human IFN-beta 1b

IFN beta 1b Human Recombinant produced in E. Coli is a single, non-glycosylated mutein (variant form) of human IFN beta-1b polypeptide chain containing 165 amino acids and having a molecular mass of 18510.86 Dalton.The IFN-beta gene was cloned from human fibroblasts and altered to substitute Serine for the Cysteine residue found at position 17.

Protein content

Protein quantitation was carried out by two independent methods:

• 1. UV spectroscopy at 280 nm using the absorbency value of 1.493 as the extinction coefficient for a 0.1% (1mg/ml) solution. The PC GENE computer analysis program of protein sequences (IntelliGenetics) calculates this value.
• 2. Analysis by RP-HPLC, using a calibrated solution of IFN-beta as a Reference Standard.

Biological Activity

The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be ≥ 3.2 x 10⁷ units/mg.

Reconstitution

We recommend a quick spin followed by reconstitution in water to a concentration of at least 100µg/ml. This solution can then be diluted into other aqueous solutions.

Stability

Lyophilized Human Interferon-beta 1b, although stable at room temperature for 3 weeks, should be stored desiccated below -20°C. Upon reconstitution, Human IFN-beta 1b should be stored at 4°C between 2-7 days and below -20°C for future use. For long term storage we recommend to add at least 0.1% HSA (order number: 2835.955.xyz or 2835.958.xyz) or BSA (order number: 2835.919.xyz). Please avoid repeated freeze/thaw cycles.

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Amino Acid Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Asn-Leu-Leu.

Shipped at room temperature

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