Human IFN-beta 1b recombinant Protein
Leukocyte IFN, B cell IFN, Type I IFN, IFNB1, IFB, IFF, IFNB, IFN-b 1b, MGC96956.
Description of Human IFN-beta 1b
IFN beta 1b Human Recombinant produced in E. coli is a single, non-glycosylated mutein (variant form) of human IFN beta-1b polypeptide chain containing 165 amino acids and having a molecular mass of 18510.86 Dalton.The IFN-beta gene was cloned from human fibroblasts and altered to substitute Serine for the Cysteine residue found at position 17.
Protein quantitation was carried out by two independent methods:
- 1. UV spectroscopy at 280 nm using the absorbency value of 1.493 as the extinction coefficient for a 0.1% (1mg/ml) solution. The PC GENE computer analysis program of protein sequences (IntelliGenetics) calculates this value.
- 2. Analysis by RP-HPLC, using a calibrated solution of IFN-beta as a Reference Standard.
The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be ≥ 3.2 x 107 units/mg.
We recommend a quick spin followed by reconstitution in water to a concentration of at least 100µg/ml. This solution can then be diluted into other aqueous solutions.
Lyophilized Human Interferon-beta 1b, although stable at room temperature for 3 weeks, should be stored desiccated below -20°C. Upon reconstitution, Human IFN-beta 1b should be stored at 4°C between 2-7 days and below -20°C for future use. For long term storage we recommend to add at least 0.1% HSA (order number: 2835.955.xyz or 2835.958.xyz) or BSA (order number: 2835.919.xyz). Please avoid repeated freeze/thaw cycles.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Asn-Leu-Leu.
Shipped at room temperature
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