Human PDGF-AB recombinant Protein

Bitte loggen Sie sich ein, um die Sonder­konditionen Ihrer Einrichtung zu sehen.

Synonyms

PDGFA, PDGF1, PDGF-A.

Description of Human PDGF-AB

PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet a-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-a and PDGFR-ß. PDGFR-a is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-ß interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AB is a 25.5 kDa disulfide-linked dimer, consisting of one A chain and one B chains (234 total amino acids).

Biological Activity

The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).

Reconstitution

Centrifuge vial prior to opening. The lyophilized PDGF-AB should be reconstituted in 50mM acetic acid to a concentration not lower than 100µg/ml. For long term storage of reconstituted protein addition of carrier protein (e.g. HSA - order number: 2835.955.xyz or 2835.958.xyz - or BSA; 0.1%) is recommended.

vergrößern

Amino Acid Sequence

Alphachain :MSIEEAVPA VCKTRTVIYE IPRSQVDPTS ANFLIWPPCV EVKRCTGCCN TSSVKCQPSR VHHRSVKVAK VEYVRKKPKL KEVQVRLEEH LECACATTSL NPDYREEDTG RPRESGKKRK RKRLKPT Be tachain:MS LGSLTIAEPA MIAECKTRTE VFEISRRLID RTNANFLVWP PCVEVQRCSG CCNNRNVQCR PTQVQLRPVQ VRKIGIVRKK PIFKKATVTL GDHLACKCET VAAARPVT

Shipped at room temperature

Download Datasheet