Human PF-4 (CXCL4) recombinant Protein

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Synonyms

CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298.

Description of Human PF-4 (CXCL4)

Recombinant Human PF-4 (CXCL4) produced in E. Coli is a 7.8 kDa single, non-glycosylated polypeptide chain containing 70 amino acids.

Biological Activity

The ED₅₀ of 5-15 µg/ml was determined by its ability to inhibit human FGF basic dependent proliferation of NR6R3T3 mouse fibroblasts.

Reconstitution

We recommend a quick spin followed by reconstitution in water to a concentration at least 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week or in working aliquots at -20°C to -80°C. Working aliquots should be at the highest practical concentration. For long term storage we recommend to add at least 0.1% HSA (order number: 2835.955.xyz or 2835.958.xyz) or BSA.

Stability

Lyophilized Human PF-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PF-4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.

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Amino Acid Sequence

The sequence of the first four N-terminal amino acids is Glu-Ala-Glu-Glu-Asp.

Shipped at room temperature

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