Porcine IL-1 beta recombinant Protein

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Synonyms

Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.

Description of Porcine IL-1 beta

Recombinant porcine Interleukin-1beta (IL-1beta) produced in E. coli is a 17.6kDa single, non-glycosylated polypeptide chain containing 153 amino acids. It is a 12-stranded beta-sheet structure as both the heparin binding growth factors and the Kunitz-type sybean trypsin inhibitors. The beta-sheets are arranged in 3 similar lobes around a central axis, 6 strand forming an anti-parallel beta-barrel. Several regions, specially the loop between strands 4 and 5, have been implicated in receptor binding. IL-1-beta is a soluble factor produced by monocytes, macrophages, and other ells which activates T-lymphocytes and potentiates their response to mitogens or antigens. IL-1 consists of two forms, IL-1 alpha and IL-1 beta which perform the same functions cut are distinct proteins. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha, IFN-alpha, IFN-beta and IFN-g and also by bacterial endotoxins, viruses, mitogens, and antigens. Oblasts IL-1 alpha and TNF-alpha induce synthesis of IL-1 beta. In pheochromocytoma cells, NGF induces the synthesis of IL1.

Biological Activity

The ED₅₀ is ≤ 5.0 ng/ml and was determined by a cell proliferation assay using murine D10S cells. Its specific activity is ≥ 2.0 x 10⁵ IU/mg.

Reconstitution

It is recommended to reconstitute the lyophilized IL 1 beta in sterile distilled H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized IL 1 beta although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL 1 beta should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA, order number: 2835.919.xyz). Please avoid repeated freeze-thaw cycles.

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Amino Acid Sequence

ANVQSMECKL QDKDHKSLVL AGPHMLKALH LLTGDLKREV VFCMSFVQGD DSNNKIPVTL GIKGKNLYLS CVMKDNTPTL QLEDIDPKRY PKRDMEKRFV FYKTEIKNRV EFESALYPNW YISTSQAEQK PVFLGNSKGR QDITDFTMEV LSP

Shipped at room temperature

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