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Human BD-2 recombinant Protein
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Protein Synonyms: hBD-2, Defensin beta 2, Skin-antimicrobial peptide 1, SAP1, DEFB4, DEFB102, DEFB2, DEFB4P. Source: E. coli Biological activity: ≥ 2 x 106 units/mg Purity: ≥ 98 % (SDS-PAGE, RP-HPLC) Physical state: Lyophilized Manufacturer: |
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Active Bioscience
Product Details
Synonyms
hBD-2, Defensin beta 2, Skin-antimicrobial peptide 1, SAP1, DEFB4, DEFB102, DEFB2, DEFB4P.
Description of Human BD-2
Recombinant Human Beta defensin-2 (BD-2) is a 41 amino acid peptide, with a molecular mass of 4,3 kDa. All Defensins are highly similar in their protein sequence. They are microbicidal and cytotoxic peptides made by neutrophils. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, six human beta-defensins have been identified: BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. Human BD-1 and Human BD-2 are monomeric, while Human BD-3 is a dimer. Defensins can act as chemoattractants towards immature dendritic cells and memory T-cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors, they are released by proteolytic cleavage of a signal sequence and, in some cases, a propeptide sequence.
Biological Activity
The ED50 of 10-100ng/ml was determined by the ability to chemoattract immature human dendritic cells.
Reconstitution
We recommend a quick spin followed by reconstitution in water to a concentration of at least 100 µg/ml, which can then be further diluted. Do not vortex. This solution can be stored at 2-8°C for up to 1 week or in working aliquots at -20°C to -80°C. Working aliquots should be at the highest practical concentration.
Stability
The lyophilized protein is stable at room temperature for up to 1 month and at least until the lot specific expiry date if kept below -18°C. Reconstituted BD-2 should be stored in working aliquots at -20°C to -80°C. Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P
Shipped at room temperature
To buy Human BD-2 please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button »Shopping List & Order Form« or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable. Human BD-2 is an alternative for the no longer available Promokine BD-2, human, recombinant (C-65250).
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Errors, changes and availability excepted.
Payment Methods
Paying by bank transfer
