Human FGF-basic, cct-premium, FGF-2 recombinant Protein

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Phone: +49 (0) 40 . 43 20 84 48 0

E-Mail: info@active-bioscience.de

 

Product Overview, Sizes and Prices

 

Protein

Synonyms: FGF2, BFGF, FGFB, HBGF-2, basic Fibroblast growth factor (bFGF), Heparin binding growth factor-2

Source: E. coli

Purity: ≥ 98 % (SDS-PAGE, silver stained)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1370.950.010 10 g 60 €
1370.950.050 50 g 180 €
1370.950.100 100 g 220 €
1370.950.200 200 g 390 €
1370.950.500 500 g 450 €
1370.950.199 1 mg 750 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

FGF2, BFGF, FGFB, HBGF-2, basic Fibroblast growth factor (bFGF), Heparin binding growth factor-2, Prostatropin

 

Description of Human FGF-basic, cct-premium, FGF-2

Recombinant human Fibroblast Growth Factor-basic (hFGF-b) is a 16.5 kDa protein containing 153 amino acid residues. Interestingly, hFGF-b contains no hydrophobic leader sequence previously thought to be required for cell secretion. Basic FGF bears 55% homology to acidic FGF and also seems to exist in three forms: the 154 amino-acid form and two other truncated versions of 146 and 131 amino acids lacking the N-terminal 9 and 24 residues. hFGF-b is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. It also exerts a potent angiogenic activity in vivo. Acidic and basic FGF compete for the binding to 125 kDa and 145 kDa receptor species. However, acidic FGF has a higher affinity for the 125 kDa species, while basic FGF has a higher affinity for the 145 kDa species. FGF receptor activation leads to the activation of MAP kinase and protein kinase C. In general FGFs induce the proliferative response in cells derived from mesoderm and neuroectoderm. It seems that basic FGF reduces the average doubling time by shortening the G1 phase of the cell cycle. Furthermore, it has been reported to induce the release of plasminogen activator by endothelial cells. One of the most potentially significant applications of FGF-b is related to its reported ability to induce angiogenesis.

 

Biological Activity

The ED50 of ≤ 0.1-2.0 ng/ml was determined by stimulation of cell proliferation by human umbilical vein endothelial cells for human FGF-basic.

 

Reconstitution

We recommend a quick spin followed by reconstitution in sterile-filtered water to a concentration of at least 0.05 mg/ml. Do not vortex. This solution can then be diluted into other aqueous buffers. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20C to -80C.

 

Stability

The lyophilized protein is stable below -20C until expiry date. Reconstituted human GM-CSF should be stored in working aliquots at -20C. Please avoid repeated freeze-thaw cycles.

 

Amino Acid Sequence

AGSITTLPAL PEDGGSGAFP PGHFKDPKRL YCKNGGFFLR IHPDGRVDGV REKSDPHIKL QLQAEERGVV SIKGVCANRY LAMKEDGRLL ASKCVTDECF FFERLESNNY NTYRSRKYTS WYVALKRTGQ YKLGSKTGPG QKAILFLPMS AKS

 

 

 

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Errors, changes and availability excepted.

 

 

Payment Methods

Paying by bank transfer

Shipping

We ship with UPS

Free shipping for orders above 500  within Germany

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