Human Flt3-Ligand recombinant Protein
Fms-Related Tyrosine Kinase 3 Ligand, Flt3 Ligand, Flt-3 Ligand, SL Cytokine.
Description of Human Flt3-Ligand
Recombinant human Flt3-ligand is a soluble 17.6 kDa polypeptide containing 155 amino acid residues. Flt3-ligand is a growth factor proliferation of early hematopoietic cells. It binds to cells expressing the tyrosine kinase receptor Flt3. Flt3-ligand, by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation. Unlike SCF, Flt3-ligand exerts no activity on mast cells. Multiple isoforms of Flt3-ligand have been identified. The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein (209a.a.). The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform.
The ED50 of ≤ 1.0 ng/ml was determined by the dose dependent stimulation of the proliferation of human AML5 cells. It corresponds to a specific activity of ≥ 1x106 units/mg.
We recommend a quick spin followed by reconstitution in distilled, sterile water to a concentration of 0.1-1.0mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for one week or -20°C for future use.
The lyophilized protein is stable for few weeks at room temperature, but best stored at -20°C. Reconstituted Flt3-ligand should be stored in working aliquots at -20°C. Please avoid repeated freeze thaw cycles.
Amino Acid Sequence
TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA
Shipped at room temperature
To buy Human Flt3-Ligand (W1400950002, W1400950010, W1400950050, W1400950100, W1400950199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button »Shopping List & Order Form« or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable. Human Flt3-Ligand is an alternative for the no longer available Promokine flt-3 Ligand, human, recombinant (E. coli) (C-67110).
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