Human Flt3-Ligand recombinant Protein

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Product Overview, Sizes and Prices

 

Protein

Synonyms: Fms-Related Tyrosine Kinase 3 Ligand, Flt3 Ligand, Flt-3 Ligand, SL Cytokine.

Source: E. coli

Biological activity: ≥ 1 x 106 IU/mg.

Purity: ≥ 98 % (SDS-PAGE, HPLC)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1400.950.002 2 µg 60 €
1400.950.010 10 µg 159 €
1400.950.050 50 µg 375 €
1400.950.100 100 µg 620 €
1400.950.199 1 mg 2.990 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

Fms-Related Tyrosine Kinase 3 Ligand, Flt3 Ligand, Flt-3 Ligand, SL Cytokine.

 

Description of Human Flt3-Ligand

Recombinant human Flt3-ligand is a soluble 17.6 kDa polypeptide containing 155 amino acid residues. Flt3-ligand is a growth factor proliferation of early hematopoietic cells. It binds to cells expressing the tyrosine kinase receptor Flt3. Flt3-ligand, by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation. Unlike SCF, Flt3-ligand exerts no activity on mast cells. Multiple isoforms of Flt3-ligand have been identified. The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein (209a.a.). The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform.

 

Biological Activity

The ED50 of ≤ 1.0 ng/ml was determined by the dose dependent stimulation of the proliferation of human AML5 cells. It corresponds to a specific activity of ≥ 1x106 units/mg.

 

Reconstitution

We recommend a quick spin followed by reconstitution in distilled, sterile water to a concentration of 0.1-1.0mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for one week or -20°C for future use.

 

Stability

The lyophilized protein is stable for few weeks at room temperature, but best stored at -20°C. Reconstituted Flt3-ligand should be stored in working aliquots at -20°C. Please avoid repeated freeze thaw cycles.

 

Amino Acid Sequence

TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA

 

 

 

 

 

Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.

 

 

To buy Human Flt3-Ligand (W1400950002, W1400950010, W1400950050, W1400950100, W1400950199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button »Shopping List & Order Form« to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable.

 

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Errors, changes and availability excepted.

 

 

Payment Methods

Paying by bank transfer

Shipping

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Free shipping for orders above 500 € within Germany

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