Human IL-1 alpha recombinant Protein

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Synonyms

Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha, IL1, IL-1A, IL1F1.

Description of Human IL-1 alpha

Recombinant human Interleukin-1 alpha (IL-1 alpha) is a 18.0 kDa protein containing 159 amino acid residues. IL-1 alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macro-phages, keratinocytes and other epithelial cells. Both IL-alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymo-cyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.

Biological Activity

The ED₅₀ of ≤ 0.001ng/ml was determined by the dose-dependent stimulation of the proliferation of murine D10S cells. It corresponds to a specific activity of ≥ 1.0 x 10⁹ units/mg.

Reconstitution

We recommend a quick spin followed by reconstitution in water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4 °C for 1 week or -20°C for future use.

Stability

The lyophilized protein is stable at room temperature for a few weeks, but best stored at -20°C. Reconstituted rhIL-1alpha should be stored in working aliquots at -20°C. Please avoid repeated freeze thaw cycles.

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Amino Acid Sequence

SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA

Shipped at room temperature

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