Human IL-4 recombinant Protein

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Synonyms

IL4, BSF1, IL-4, BCGF1, BSF-1, BCGF-1, B cell Stimulating Factor.

Description of Human IL-4

Recombinant human Interleukin-4, is a 14.9 kDa globular protein containing 130 amino acid residues. IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy.

Biological Activity

The ED₅₀ of ≤ 0.25 ng/ml was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. It corresponds to a specific activity of ≥ 4 x 10⁶ units/mg.

Reconstitution

We recommend a quick spin followed by reconstitution in water to a concentration of at least 0.1mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.

Stability

The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted IL-4 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Please avoid repeated freeze-thaw cycles.

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Amino Acid Sequence

MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS

Shipped at room temperature

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